Antibody LC–MS Analysis

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Antibody LC–MS Analysis
Antibody LC–MS (Liquid Chromatography–Mass Spectrometry) analysis is a powerful analytical approach for the detailed characterization of monoclonal antibodies. By combining high-resolution chromatographic separation with sensitive mass detection, LC–MS enables comprehensive evaluation of antibody quality, and critical quality attributes.
As antibodies are large and structurally complex biomolecules (~150 kDa) with multiple post-translational modifications, robust analytical tools are essential to ensure their efficacy, and consistency. LC–MS has emerged as a gold-standard technique for antibody characterization across discovery, development, and manufacturing stages.

Key Capabilities
Our antibody LC–MS analysis service provides in-depth insights into:
• Intact mass analysis for molecular weight confirmation
• Heavy chain and light chain analysis
• Glycosylation profiling including glycoform distribution
• Post-translational modifications (PTMs) such as oxidation, deamidation
• Terminal modifications (C-terminal lysine truncation, N-terminal pyroglutamate)
These attributes are critical for evaluating antibody quality, as even minor variations can impact biological activity and clinical performance.

Analytical Approaches
We offer flexible LC–MS workflows tailored to your project requirements:
• Direct characterization of intact antibodies for rapid mass confirmation
• Heavy chain and light chain for enhanced resolution and simplified data interpretation
• Peptide mapping for detailed sequence and modification analysis
Each approach is selected based on the level of detail required and the specific analytical question being addressed.

Applications
Our LC–MS antibody analysis supports a wide range of applications, including:
• Candidate screening and clone selection
• Biosimilar comparability studies
• Process development and optimization
• Quality control and batch release testing
• Antibody-drug conjugate (ADC) characterization, including conjugation and modification analysis
LC–MS is particularly valuable for ADCs, where it enables assessment of conjugation sites, drug-to-antibody ratio (DAR), and structural integrity.

Why Choose Our Service
• High-resolution, accurate mass data for confident characterization
• Customized workflows based on your antibody format and project goals
• Fast turnaround time with clear, actionable reports
• Expert data interpretation to support decision-making

How it works
1. Sample Submission – Contact us and provide purified antibody or conjugate samples.
2. Sample Preparation – We will optimize the methods for intact analysis, heavy chain – light chain analysis and PTM analysis.
3. LC–MS Analysis – High-resolution data acquisition using advanced instrumentation.
4. Data Processing & Reporting – Comprehensive report with key quality attributes and interpretation.